OrangerV1, Main, Exploration, bibRecord, 003377

Cryopreservation and storage of embryogenic callus cultures of several Citrus species and cultivars

Identifieur interne : 003377 ( Main/Exploration ); précédent : 003376; suivant : 003378

Cryopreservation and storage of embryogenic callus cultures of several Citrus species and cultivars

Auteurs : R. M. Perez [Espagne] ; L. Navarro [Espagne] ; N. Duran-Vila [Espagne]

Source :

Plant cell reports [ 0721-7714 ] ; 1997.

RBID : Pascal:98-0058421

Descripteurs français

Pascal (Inist)
- Embryon, Stockage cryogénique, Gel dégel, Culture embryon, Citrus paradisi, Citrus limon, Citrus aurantium, Citrus aurantifolia, Citrus sinensis, Cryoconservation, Cal, Développement embryonnaire, Evaluation performance, Végétal, Agrume, Germoplasme.
Wicri :
- topic : Agrume.

English descriptors

KwdEn :
- Callus, Citrus aurantifolia, Citrus aurantium, Citrus fruit, Citrus limon, Citrus paradisi, Citrus sinensis, Cryogenic storage, Cryopreservation, Embryo, Embryo culture, Embryonic development, Freeze thaw cycle, Germplasm, Performance evaluation, Vegetals.

Abstract

Nucellus-derived embryogenic callus cultures of Salustiana sweet orange were subjected to cryoconser-vation assays. Cryoprotection with 10%(vol/vol) dimethylsulfoxide, freezing by slow cooling and thawing by fast warming was suitable to recover viable growing cultures and whole plants through embryogenesis. Evaluation of liquid phase R₁ and solid phase R₂ cooling rates using a programmable freezing unit indicated that 100% of embryogenic cultures survived when frozen using a range of cooling rates (R₁ not above 0.5°C min^-1 and R₂ not above 1°C min¹) and thawed by fast warming. Storage up to 2 years in liquid nitrogen did not affect the growth of the cryopreserved cultures and the recovery of whole plants. Cultures of four cultivars of sweet orange (C. sinensis Osb.), three cultivars of grapefruit (C. paradisi Macf.), and one cultivar each of lemon [C. limon (L.) Burm. f.], Cleo-patra mandarin (C. reshni Hort. ex Tan.), sour orange (C. aurantium L.) and Mexican lime [C. aurantifolia (Christm.) Swing.] have been successfully cryopreserved. Problems using a viability assessment using fluorescein diacetate staining are discussed.

Affiliations:

Links toward previous steps (curation, corpus...)

to stream PascalFrancis, to step Corpus: 000B02
to stream PascalFrancis, to step Curation: 001405
to stream PascalFrancis, to step Checkpoint: 000B21
to stream Main, to step Merge: 003627
to stream Main, to step Curation: 003377

Le document en format XML

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<s2>46113 Moncada (Valencia)</s2>
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<author><name sortKey="Navarro, L" sort="Navarro, L" uniqKey="Navarro L" first="L." last="Navarro">L. Navarro</name>
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<author><name sortKey="Duran Vila, N" sort="Duran Vila, N" uniqKey="Duran Vila N" first="N." last="Duran-Vila">N. Duran-Vila</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Cryopreservation and storage of embryogenic callus cultures of several Citrus species and cultivars</title>
<author><name sortKey="Perez, R M" sort="Perez, R M" uniqKey="Perez R" first="R. M." last="Perez">R. M. Perez</name>
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<s2>46113 Moncada (Valencia)</s2>
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<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
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<author><name sortKey="Duran Vila, N" sort="Duran Vila, N" uniqKey="Duran Vila N" first="N." last="Duran-Vila">N. Duran-Vila</name>
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<s2>46113 Moncada (Valencia)</s2>
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<term>Citrus aurantifolia</term>
<term>Citrus aurantium</term>
<term>Citrus fruit</term>
<term>Citrus limon</term>
<term>Citrus paradisi</term>
<term>Citrus sinensis</term>
<term>Cryogenic storage</term>
<term>Cryopreservation</term>
<term>Embryo</term>
<term>Embryo culture</term>
<term>Embryonic development</term>
<term>Freeze thaw cycle</term>
<term>Germplasm</term>
<term>Performance evaluation</term>
<term>Vegetals</term>
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<keywords scheme="Pascal" xml:lang="fr"><term>Embryon</term>
<term>Stockage cryogénique</term>
<term>Gel dégel</term>
<term>Culture embryon</term>
<term>Citrus paradisi</term>
<term>Citrus limon</term>
<term>Citrus aurantium</term>
<term>Citrus aurantifolia</term>
<term>Citrus sinensis</term>
<term>Cryoconservation</term>
<term>Cal</term>
<term>Développement embryonnaire</term>
<term>Evaluation performance</term>
<term>Végétal</term>
<term>Agrume</term>
<term>Germoplasme</term>
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<keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Agrume</term>
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<front><div type="abstract" xml:lang="en">Nucellus-derived embryogenic callus cultures of Salustiana sweet orange were subjected to cryoconser-vation assays. Cryoprotection with 10%(vol/vol) dimethylsulfoxide, freezing by slow cooling and thawing by fast warming was suitable to recover viable growing cultures and whole plants through embryogenesis. Evaluation of liquid phase R<sub>1</sub>
 and solid phase R<sub>2</sub>
 cooling rates using a programmable freezing unit indicated that 100% of embryogenic cultures survived when frozen using a range of cooling rates (R<sub>1</sub>
 not above 0.5°C min<sup>-1</sup>
 and R<sub>2</sub>
 not above 1°C min<sup>1</sup>
) and thawed by fast warming. Storage up to 2 years in liquid nitrogen did not affect the growth of the cryopreserved cultures and the recovery of whole plants. Cultures of four cultivars of sweet orange (C. sinensis Osb.), three cultivars of grapefruit (C. paradisi Macf.), and one cultivar each of lemon [C. limon (L.) Burm. f.], Cleo-patra mandarin (C. reshni Hort. ex Tan.), sour orange (C. aurantium L.) and Mexican lime [C. aurantifolia (Christm.) Swing.] have been successfully cryopreserved. Problems using a viability assessment using fluorescein diacetate staining are discussed.</div>
</front>
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<affiliations><list><country><li>Espagne</li>
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<tree><country name="Espagne"><region name="Communauté valencienne"><name sortKey="Perez, R M" sort="Perez, R M" uniqKey="Perez R" first="R. M." last="Perez">R. M. Perez</name>
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<name sortKey="Duran Vila, N" sort="Duran Vila, N" uniqKey="Duran Vila N" first="N." last="Duran-Vila">N. Duran-Vila</name>
<name sortKey="Navarro, L" sort="Navarro, L" uniqKey="Navarro L" first="L." last="Navarro">L. Navarro</name>
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Cryopreservation and storage of embryogenic callus cultures of several Citrus species and cultivars

Cryopreservation and storage of embryogenic callus cultures of several Citrus species and cultivars

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri